VITRIA BUDIYANTI. K2D006104. Potential of bacteria of seagrass Halophila ovalis as a antifoulant producer from Legonlele Karimunjawa Jepara. (Ocky Karna Radjasa and Agus Sabdono).
The rapid growth of development, making coastal areas as important areas for various development projects, such as the construction of docks, ports, power plant, as well as other coastal buildings, which generally have parts that always flooded by sea water. So far biofouling has been controlled by the using of chemical-based antifoulants, which are believed to create another environmental problem. One alternative that can be offered is the use of bacterial symbionts of seagrass ( seagrass ), particularly Halophila ovalis.
The purpose of this study are to perform bacterial isolation and screening of symbionts of the seagrass H. ovalis that have potential in producing antifouling compounds against tested biofilm-forming bacteria, which are expected to provide an alternative source as an environmentally friendly antifoulants.
The study was conducted from the month September 2008 - June 2009. Sampling was conducted in Legonlele waters, Karimunjawa, Jepara. Preparation of primary biofilms ( wooden and fiber panels ) was also conducted in the Legonlele waters, Karimunjawa, Jepara. Isolation of bacteria and testing activities were conducted at the integrated Laboratory of Marine Science UNDIP, Semarang. Moleculer works were carried out at the Laboratory of Molecular Biotechnology Faculty of Agriculture UGM Yogyakarta. Whereas DNA sequencing was performed at the Laboratory of UIN, Yogyakarta.
The results showed that from seventeen bacterial symbionts of H. ovalis only two bacteria showed antifouling activity against bacterial biofilms. Molecular test showed that two active bacteria were LLH 6, which is closely related to Bacteroidetes bacterium with high homology of 97 %, while another isolate, LHT 2, is mostly affiliated with Marine sponge bacterium ( 99 % ). On the other hand bacterial biofilms FLHH 1 and KLHH 2 were closely related to Virgibacillus salarius with homology of 98 %, respectively.
Keywords: Seagrass, Biofouling, 16S r DNA PCR, Halophila ovalis
Contact Person:
VITRIA BUDIYANTI
v.budiyanti@yahoo.com
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